2FD6

Structure of Human Urokinase Plasminogen Activator in Complex with Urokinase Receptor and an anti-upar antibody at 1.9 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	90
Detector technology	CCD
Collection date	2005-04-09
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	51.792, 86.805, 124.690
Unit cell angles	90.00, 94.54, 90.00

Refinement procedure

Resolution	41.360 - 1.900
R-factor	0.239
Rwork	0.239
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fat
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.360	2.080
High resolution limit [Å]	1.850	1.850
Rmerge	0.064	0.338
Number of reflections	86852
<I/σ(I)>	16.1	1.97
Completeness [%]	94.5	77.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.5	298	4% PEG4000, 5% ethylene glycol, 5% methanol, 0.05% sodium azide, 50 mM cacodylate, pH 6.5, MICRODIALYSIS, temperature 298K
1	MICRODIALYSIS	6.5	298	4% PEG4000, 5% ethylene glycol, 5% methanol, 0.05% sodium azide, 50 mM cacodylate, pH 6.5, MICRODIALYSIS, temperature 298K
1	MICRODIALYSIS	6.5	298	4% PEG4000, 5% ethylene glycol, 5% methanol, 0.05% sodium azide, 50 mM cacodylate, pH 6.5, MICRODIALYSIS, temperature 298K
1	MICRODIALYSIS	6.5	298	4% PEG4000, 5% ethylene glycol, 5% methanol, 0.05% sodium azide, 50 mM cacodylate, pH 6.5, MICRODIALYSIS, temperature 298K