2E59
Crystal structure of human MD-2 in complex with lipid IVa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-10 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.795, 52.795, 110.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.720 - 2.210 |
| R-factor | 0.20842 |
| Rwork | 0.206 |
| R-free | 0.26326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e56 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.720 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.100 |
| Number of reflections | 7629 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 90.7 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 15% PEG3350, 40mM Na-citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
