2DSQ
Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.280, 43.660, 81.150 |
Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.29188 |
Rwork | 0.288 |
R-free | 0.35708 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dsp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 13980 | |
Completeness [%] | 99.0 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 290 | 20% PEG 3350, 0.2M lithium acetate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 290K |