2BFX
Mechanism of Aurora-B activation by INCENP and inhibition by Hesperadin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 287 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.940, 66.900, 116.520 |
| Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.019 |
| Number of reflections | 64766 | |
| <I/σ(I)> | 29.3 | 8.4 |
| Completeness [%] | 99.3 | 94.4 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PROPANE, 18% PEG3350 |
