2BBZ
Crystal Structure of MC159 Reveals Molecular Mechanism of DISC Assembly and vFLIP Inhibition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-24 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 83.857, 108.065, 142.643 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.800 |
R-factor | 0.325 |
Rwork | 0.325 |
R-free | 0.37200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.273 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 13309 | |
Completeness [%] | 99.7 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 50-150 mM sodium/potassium phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |