2B2X
VLA1 RdeltaH I-domain complexed with a quadruple mutant of the AQC2 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 106.120, 43.680, 153.880 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mhp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.366 |
| Number of reflections | 66690 | |
| Completeness [%] | 94.2 | |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Peg 20K, Mes, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
