2AZE
Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9498 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 146.800, 168.600, 48.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.550 |
Rwork | 0.221 |
R-free | 0.26200 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.157 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 19350 | |
Completeness [%] | 97.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Sodium Citrate, Ammonium Sulfate, PEG 400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Sodium Citrate, Ammonium Sulfate, PEG 400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |