2AS5
Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1070 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.455, 157.447, 67.666 |
Unit cell angles | 90.00, 118.67, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.2382 |
Rwork | 0.238 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a02 chain N |
RMSD bond length | 0.008 |
RMSD bond angle | 1.306 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.085 | |
Number of reflections | 31620 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 96.0 | 86.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | Cacodylic Acid, PEG 4k, Sodium Chloride, Magnesium Chloride, Glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cacodylic Acid | ||
10 | 1 | 2 | Magnesium Chloride | ||
2 | 1 | 1 | PEG 4k | ||
3 | 1 | 1 | Sodium Chloride | ||
4 | 1 | 1 | Magnesium Chloride | ||
5 | 1 | 1 | Glycerol | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | Cacodylic Acid | ||
8 | 1 | 2 | PEG 4k | ||
9 | 1 | 2 | Sodium Chloride |