2AG9
Crystal Structure of the Y137S mutant of GM2-Activator Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2004-07-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92015 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.490, 48.390, 55.590 |
| Unit cell angles | 76.16, 87.70, 87.54 |
Refinement procedure
| Resolution | 19.870 - 2.200 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ag4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.291 |
| Number of reflections | 18838 | |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 66.6 | 26 |
| Redundancy | 1.7 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 278 | Peg 4000, Hepes buffer, isopropanol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
