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2AG9

Crystal Structure of the Y137S mutant of GM2-Activator Protein

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]123
Detector technologyCCD
Collection date2004-07-30
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.92015
Spacegroup nameP 1
Unit cell lengths41.490, 48.390, 55.590
Unit cell angles76.16, 87.70, 87.54
Refinement procedure
Resolution19.870 - 2.200
R-factor0.243
Rwork0.243
R-free0.33400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2ag4
RMSD bond length0.010
RMSD bond angle1.500
Data reduction softwareHKL-2000
Data scaling softwareCCP4 ((TRUNCATE))
Phasing softwareCNS
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.070
High resolution limit [Å]2.0002.000
Rmerge0.0820.291
Number of reflections18838
<I/σ(I)>9.91.7
Completeness [%]66.626
Redundancy1.71.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.9278Peg 4000, Hepes buffer, isopropanol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 278K

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