2AG9
Crystal Structure of the Y137S mutant of GM2-Activator Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2004-07-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.92015 |
Spacegroup name | P 1 |
Unit cell lengths | 41.490, 48.390, 55.590 |
Unit cell angles | 76.16, 87.70, 87.54 |
Refinement procedure
Resolution | 19.870 - 2.200 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.33400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ag4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.291 |
Number of reflections | 18838 | |
<I/σ(I)> | 9.9 | 1.7 |
Completeness [%] | 66.6 | 26 |
Redundancy | 1.7 | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 278 | Peg 4000, Hepes buffer, isopropanol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 278K |