2A5F
Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-08 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.905, 90.925, 98.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 2.020 |
| R-factor | 0.18369 |
| Rwork | 0.181 |
| R-free | 0.23075 |
| Structure solution method | Isomorphous replacement |
| Starting model (for MR) | CTA1:ARF6-GTP |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 25861 | |
| Completeness [%] | 97.5 | 81.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG2000mme, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
