2A4Z
Crystal Structure of human PI3Kgamma complexed with AS604850
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0716 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.352, 67.648, 106.390 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.900 |
| R-factor | 0.264 |
| Rwork | 0.257 |
| R-free | 0.35200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 106.000 | 19.820 | 3.060 |
| High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
| Rmerge | 0.087 | 0.058 | 0.314 |
| Number of reflections | 21670 | ||
| <I/σ(I)> | 6.4 | 11.1 | 2.1 |
| Completeness [%] | 96.1 | ||
| Redundancy | 4.2 | 3.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 22% PEG 4K, 100mM Tris-HCl, 200mM (NH4)2SO4, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
