2A4Z

Crystal Structure of human PI3Kgamma complexed with AS604850

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-05
Detector	MARRESEARCH
Wavelength(s)	1.0716
Spacegroup name	C 1 2 1
Unit cell lengths	142.352, 67.648, 106.390
Unit cell angles	90.00, 95.77, 90.00

Refinement procedure

Resolution	19.750 - 2.900
R-factor	0.264
Rwork	0.257
R-free	0.35200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8y
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.000	19.820	3.060
High resolution limit [Å]	2.900	9.170	2.900
Rmerge	0.087	0.058	0.314
Number of reflections	21670
<I/σ(I)>	6.4	11.1	2.1
Completeness [%]	96.1
Redundancy	4.2	3.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	300	22% PEG 4K, 100mM Tris-HCl, 200mM (NH4)2SO4, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 300K