2A25
Crystal structure of Siah1 SBD bound to the peptide EKPAAVVAPITTG from SIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.635, 107.761, 78.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.200 |
| R-factor | 0.21529 |
| Rwork | 0.213 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k2f chain A |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.554 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.043 | 0.365 |
| Number of reflections | 10567 | |
| <I/σ(I)> | 27 | 4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | sodium dihydrogen phospahte, di-potassium hydrogen phosphate, dithiothreithol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
