2Y4I
KSR2-MEK1 heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 130.565, 130.565, 221.005 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.860 - 3.460 |
| R-factor | 0.2327 |
| Rwork | 0.230 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1S9J 2p55 1uwh AND 3C4C |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.729 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CaspR |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.650 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.093 | 0.590 |
| Number of reflections | 25175 | |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 12% (W/V) PEG 3350, 0.2 M NA CITRATE, 10 MM PR ACETATE, BIS-TRIS PROPANE PH 6.25. |
