2XWT
CRYSTAL STRUCTURE OF THE TSH RECEPTOR IN COMPLEX WITH A BLOCKING TYPE TSHR AUTOANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.020, 89.300, 101.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.980 - 1.900 |
| R-factor | 0.17943 |
| Rwork | 0.177 |
| R-free | 0.22674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g04 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.344 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 56573 | |
| <I/σ(I)> | 11.7 | 3.5 |
| Completeness [%] | 94.5 | 64.8 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 16% PEG 3350, 0.2 M SODIUM MALONATE PH 5. |
