2X1W
Crystal Structure of VEGF-C in Complex with Domains 2 and 3 of VEGFR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.750, 123.830, 211.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.958 - 2.700 |
| R-factor | 0.2297 |
| Rwork | 0.227 |
| R-free | 0.27980 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX (CDE IMPLEMENTED IN HKL2MAP) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.730 |
| Number of reflections | 53953 | |
| <I/σ(I)> | 20.1 | 2.8 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 100 MM MES BUFFER, PH 5.0-5.6, 50 MM CSCL AND 28-32 % (W/V) JEFFAMINE 600. |
