2VFX

Structure of the Symmetric Mad2 Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-16
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	109.337, 191.407, 154.307
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	45.000 - 1.950
R-factor	0.209
Rwork	0.208
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1klq
RMSD bond length	0.011
RMSD bond angle	1.501
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	0.550
Number of reflections	223558
<I/σ(I)>	14.9	1.6
Completeness [%]	97.7	95.7
Redundancy	2.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	VAPOR DIFFUSION, HANGING DROP, 20 DEGREES C. 1 MICROLITER PROTEIN: 3 MG/ML IN 20 MM TRIS, PH 8.0, 50 MM NACL, 2 MM TCEP PLUS 1 MICROLITER RESERVOIR: 19% PEG2000, 16% GLYCEROL, 100 MM TRIS, PH 8.0, 0.3 M MGCL2