2VDY
Crystal structure of the reactive loop cleaved Corticosteroid Binding Globulin complexed with Cortisol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.673, 39.757, 111.586 |
| Unit cell angles | 83.09, 85.70, 69.42 |
Refinement procedure
| Resolution | 19.430 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 22575 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 93.5 | 91.1 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 10-20% PEG3350, 0.2M NACL, pH 7.4 |
