2PE4

Structure of Human Hyaluronidase 1, a Hyaluronan Hydrolyzing Enzyme Involved in Tumor Growth and Angiogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	92.050, 92.050, 143.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.650 - 2.000
R-factor	0.19
Rwork	0.190
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fcu
RMSD bond length	0.013
RMSD bond angle	1.823
Data reduction software	d*TREK
Data scaling software	d*TREK (8.0SSI)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.650	30.650	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.063	0.044	0.488
Total number of observations		56516	40866
Number of reflections	47955
<I/σ(I)>	14	33.4	3.4
Completeness [%]	99.4	99.6	97.7
Redundancy	10.48	10.95	8.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	1.5 M sodium chloride, 10% ethanol, 3% galactose, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K