2NZ6
Crystal structure of the PTPRJ inactivating mutant C1239S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 88.521, 88.521, 118.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.980 - 2.300 |
| R-factor | 0.18399 |
| Rwork | 0.182 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cfv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.980 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.387 |
| Number of reflections | 15274 | |
| Completeness [%] | 99.2 | 95 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.01M NiCl2, 0.1M TRIS, pH 8.5, 1M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
