2NPA
the crystal structure of the human PPARaplpha ligand binding domain in complex with a a-hydroxyimino phenylpropanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-20 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.909, 100.074, 61.311 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.220 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k7l |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.180 | 2.300 |
| Rmerge | 0.059 | 0.030 | 0.376 |
| Number of reflections | 29916 | ||
| <I/σ(I)> | 18.1 | ||
| Completeness [%] | 95.9 | 85.1 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 6% PEG 10K. 0.1M Hepes, 6% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
