2JBU
Crystal structure of human insulin degrading enzyme complexed with co- purified peptides.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 263.277, 263.277, 90.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.860 - 3.000 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g47 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 72886 | |
| <I/σ(I)> | 19.9 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
