2I6B
Human Adenosine Kinase in Complex with An Acetylinic Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 110 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.180, 58.200, 89.210 |
Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.221 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i6a |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | BUSTER |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.068 |
Number of reflections | 25799 |
Completeness [%] | 87.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG4000, 0.1 M MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |