2DSQ
Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.280, 43.660, 81.150 |
| Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.29188 |
| Rwork | 0.288 |
| R-free | 0.35708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dsp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 13980 | |
| Completeness [%] | 99.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 290 | 20% PEG 3350, 0.2M lithium acetate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
