2BBZ
Crystal Structure of MC159 Reveals Molecular Mechanism of DISC Assembly and vFLIP Inhibition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 83.857, 108.065, 142.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.800 |
| R-factor | 0.325 |
| Rwork | 0.325 |
| R-free | 0.37200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.273 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 13309 | |
| Completeness [%] | 99.7 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 50-150 mM sodium/potassium phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
