2AZE

Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9498
Spacegroup name	C 2 2 21
Unit cell lengths	146.800, 168.600, 48.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.550
Rwork	0.221
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	19350
Completeness [%]	97.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	Sodium Citrate, Ammonium Sulfate, PEG 400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	Sodium Citrate, Ammonium Sulfate, PEG 400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K