2AS5
Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1070 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.455, 157.447, 67.666 |
| Unit cell angles | 90.00, 118.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.2382 |
| Rwork | 0.238 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a02 chain N |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.085 | |
| Number of reflections | 31620 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 96.0 | 86.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | Cacodylic Acid, PEG 4k, Sodium Chloride, Magnesium Chloride, Glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Cacodylic Acid | ||
| 10 | 1 | 2 | Magnesium Chloride | ||
| 2 | 1 | 1 | PEG 4k | ||
| 3 | 1 | 1 | Sodium Chloride | ||
| 4 | 1 | 1 | Magnesium Chloride | ||
| 5 | 1 | 1 | Glycerol | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | Cacodylic Acid | ||
| 8 | 1 | 2 | PEG 4k | ||
| 9 | 1 | 2 | Sodium Chloride |
