1XSP
Crystal Structure of human DNA polymerase lambda in complex with nicked DNA and pyrophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.845, 62.758, 140.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.720 - 2.200 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 25700 | |
<I/σ(I)> | 16.23 | |
Completeness [%] | 98.6 | 91.8 |
Redundancy | 6.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 2-propanol, sodium citrate, sodium chloride, cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 2-propanol | ||
2 | 1 | 1 | sodium citrate | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | cacodylate |