1XFA
Structure of NBD1 from murine CFTR- F508R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 139.994, 139.994, 278.721 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.270 - 3.100 |
| R-factor | 0.25 |
| Rwork | 0.249 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ROX monomer stripped of heterogens |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 25581 | |
| <I/σ(I)> | 34.8 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.9 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
