1X86
Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF in complex with RhoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.069 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 296.418, 95.239, 157.338 |
Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.220 |
R-factor | 0.22958 |
Rwork | 0.227 |
R-free | 0.27943 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.606 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 60392 | |
Completeness [%] | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | PEG8K, sodium phosphate, sodium chloride, EDTA, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |