1TV6
HIV-1 Reverse Transcriptase Complexed with CP-94,707
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 223.910, 69.040, 104.330 |
Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.31200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3hvt |
RMSD bond length | 0.096 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.058 | |
Number of reflections | 37716 | |
<I/σ(I)> | 29.1 | 1.6 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 50 mM bis-Tris-propane, 100 mM ammonium sulfate, 0.2% (w/v) beta-octylglucoside, 10% (v/v) glycerol, 14% (w/v) PEG-8000, pH 7.0 |