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1TK5

T7 DNA polymerase binary complex with 8 oxo guanosine in the templating strand

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
Collection date2003-11-23
DetectorADSC QUANTUM 4
Wavelength(s)1.1
Spacegroup nameP 21 21 2
Unit cell lengths105.310, 213.265, 52.181
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution26.330 - 2.200
R-factor0.214
Rwork0.214
R-free0.24600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1t7p
RMSD bond length0.006
RMSD bond angle1.100
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.110
High resolution limit [Å]2.0002.000
Number of reflections79532
<I/σ(I)>17.9
Completeness [%]99.699.6
Redundancy43
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5PEG 8000, Ammonium Sulfate. PEG400, Etylen Glyco, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K

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