1RR8
Structural Mechanisms of Camptothecin Resistance by Mutations in Human Topoisomerase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.590, 115.585, 75.010 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.600 |
| R-factor | 0.292 |
| Rwork | 0.242 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4t |
| RMSD bond length | 0.054 |
| RMSD bond angle | 2.200 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.020 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 27855 | |
| Completeness [%] | 92.9 | 81.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG 8000, 200mM Lithium Sulfate, 100mM Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG | ||
| 2 | 1 | 1 | LiSO4 | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 2 | PEG | ||
| 5 | 1 | 2 | LiSO4 |
