1RC1
Human GAR Tfase complex structure with polyglutamated 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | ADSC QUANTUM 9 |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.664, 126.664, 93.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.140 - 2.250 |
| R-factor | 0.23483 |
| Rwork | 0.234 |
| R-free | 0.25591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1njs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 41237 | |
| <I/σ(I)> | 25.5 | 2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 4.08 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | MPD, Calcium cloride, imidazole malate buffer, pH 6., VAPOR DIFFUSION, SITTING DROP, temperature 277K |
