1PZM
Crystal structure of HGPRT-ase from Leishmania tarentolae in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.53700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.104, 85.443, 87.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.100 |
| R-factor | 0.17549 |
| Rwork | 0.173 |
| R-free | 0.21525 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc1 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.09) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 24231 | |
| Completeness [%] | 92.8 | 96.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, i-propanol, glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
