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1TC1

A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI

Summary for 1TC1
Entry DOI10.2210/pdb1tc1/pdb
DescriptorPROTEIN (HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE), FORMYCIN B, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
Functional Keywordstransferase, phosphoribosyltransferase, purine salvage, nucleotide metabolism
Biological sourceTrypanosoma cruzi
Cellular locationCytoplasm : Q27796
Total number of polymer chains2
Total formula weight51855.34
Authors
Focia, P.J.,Craig III, S.P.,Nieves-Alicea, R.,Fletterick, R.J.,Eakin, A.E. (deposition date: 1998-09-30, release date: 1999-10-07, Last modification date: 2023-08-23)
Primary citationFocia, P.J.,Craig III, S.P.,Nieves-Alicea, R.,Fletterick, R.J.,Eakin, A.E.
A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi.
Biochemistry, 37:15066-15075, 1998
Cited by
PubMed Abstract: The hypoxanthine phosphoribosyltransferase (HPRT) from Trypanosoma cruzi, etiologic agent of Chagas' disease, was cocrystallized with the inosine analogue Formycin B (FmB) and the structure determined to 1.4 A resolution. This is the highest resolution structure yet reported for a phosphoribosyltransferase (PRT), and the asymmetric unit of the crystal contains a dimer of closely associated, nearly identical subunits. A conserved nonproline cis peptide in one active-site loop exposes the main-chain nitrogen to the enzyme active site, while the adjacent lysine side chain interacts with the other subunit of the dimer, thereby providing a possible mechanism for communication between the subunits and their active sites. The three-dimensional coordinates for the invariant Ser103-Tyr104 dipeptide are reported here for the first time. These are the only highly conserved residues in a second active-site loop, termed the long flexible loop, which is predicted to close over the active site of HPRTs to protect a labile transition state [Eads et al. (1994) Cell 78, 325-334]. This structure represents a major step forward in efforts to design/discover potent selective inhibitors of the HPRT of T. cruzi.
PubMed: 9790669
DOI: 10.1021/bi981052s
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.41 Å)
Structure validation

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