1TC1
A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.500, 94.090, 52.120 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.410 |
| R-factor | 0.193 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hgx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.850 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.440 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.061 | 0.196 |
| Number of reflections | 71678 | |
| <I/σ(I)> | 21.3 | 4.7 |
| Completeness [%] | 98.0 | 81.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.75 | pH 5.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | FmB | 5-10 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 15-20 (%) | |
| 4 | 1 | reservoir | MES | 0.1 (M) |
