1P17
Hypoxanthine Phosphoribosyltransferase from Trypanosoma cruzi, K68R mutant, complexed with the product IMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 157.315, 122.878, 52.121 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.760 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.104 | 0.474 |
Number of reflections | 28171 | |
<I/σ(I)> | 12.1 | 2.5 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | PEG 6000, Sodium Acetate, Ammonium Acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |