1NW3
Structure of the Catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 152.800, 152.800, 50.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.059 |
| Total number of observations | 175389 * |
| Number of reflections | 23800 * |
| <I/σ(I)> | 18.3 |
| Completeness [%] | 96.3 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 290 | Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
| 1 | Vapor diffusion * | 8 * | 290 | Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | SAM | 3 (mM) | |
| 7 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.3-1.9 (M) | |
| 9 | 1 | reservoir | TCEP | 1-3 (mM) |
