1MU2

CRYSTAL STRUCTURE OF HIV-2 REVERSE TRANSCRIPTASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	149.770, 107.810, 82.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.350
R-factor	0.189 *
Rwork	0.192
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hmv 1rtj
RMSD bond length	0.008
RMSD bond angle	1.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.128	0.747
Total number of observations	631721 *
Number of reflections	56142	5520 *
<I/σ(I)>	16	1.1
Completeness [%]	100.0	100
Redundancy	11.3	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.5	294	Bird, L.E., (2003) Protein EExpression Purif., 27, 12. *
1	unknown *	8.5	294	Bird, L.E., (2003) Protein EExpression Purif., 27, 12. *