1LM8
Structure of a HIF-1a-pVHL-ElonginB-ElonginC Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.500, 59.500, 245.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vcb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.079 | 0.382 |
| Total number of observations | 248351 * | |
| Number of reflections | 34115 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 88.7 | 76.5 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 25 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 21 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane | 5 (mM) | pH7.0 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PME5000 | 24 (%(w/v)) | |
| 6 | 1 | reservoir | potassium phosphate | 0.1 (M) | pH6.5 |
| 7 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
