1LBC
Crystal structure of GluR2 ligand binding core (S1S2J-N775S) in complex with cyclothiazide (CTZ) as well as glutamate at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9196 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.859, 162.571, 47.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.800 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.24300 * |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 * | 0.346 * |
| Number of reflections | 81971 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | pEG 8000 Zn(oAc)2, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 13-18 (%) | |
| 3 | 1 | reservoir | 0.1-0.25 (M) | ||
| 4 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.5 |
