1KZ7
Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.500, 87.980, 230.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.400 |
R-factor | 0.197 * |
Rwork | 0.197 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.230 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.089 * | 0.433 * |
Total number of observations | 418244 * | |
Number of reflections | 49528 * | |
Completeness [%] | 90.7 | 58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 18 * | PEG 8000, sodium formate, Tris HCL, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
3 | 1 | drop | 25 (mM) | ||
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | reservoir | Tris | 50 (mM) | pH7.0 |
7 | 1 | reservoir | PEG8000 | 12 (%) | |
8 | 1 | reservoir | sodium formate | 650 (mM) | |
9 | 1 | reservoir | dithiothreitol | 2 (mM) |