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1KZ7

Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyCCD
Collection date2000-02-15
DetectorADSC QUANTUM 4
Spacegroup nameP 21 21 21
Unit cell lengths66.500, 87.980, 230.510
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000

*

- 2.400
R-factor0.197

*

Rwork0.197
R-free0.23900
Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle1.230

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHELXS
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.000

*

2.490
High resolution limit [Å]2.4002.400
Rmerge0.089

*

0.433

*

Total number of observations418244

*

Number of reflections49528

*

Completeness [%]90.758
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

8

*

18

*

PEG 8000, sodium formate, Tris HCL, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein14 (mg/ml)
21dropTris10 (mM)pH8.0
31drop25 (mM)
41dropdithiothreitol2 (mM)
51dropEDTA1 (mM)
61reservoirTris50 (mM)pH7.0
71reservoirPEG800012 (%)
81reservoirsodium formate650 (mM)
91reservoirdithiothreitol2 (mM)

217705

PDB entries from 2024-03-27

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