1K5D
Crystal structure of Ran-GPPNHP-RanBP1-RanGAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.551, 103.109, 120.177 |
| Unit cell angles | 71.59, 80.55, 67.78 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.238 * |
| Rwork | 0.237 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.122 | 0.235 |
| Total number of observations | 409414 * | |
| Number of reflections | 163530 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 97.6 | 97.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG4000, POTASSIUM ACETATE, TRIS-HCL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18.5-20.5 (%) | |
| 2 | 1 | reservoir | potassium acetate | 100 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 5 | 1 | drop | 2 (mM) | ||
| 6 | 1 | drop | DTE | 2 (mM) |
