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1K3A

Structure of the Insulin-like Growth Factor 1 Receptor Kinase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]100
Detector technologyCCD
Collection date2001-05-22
DetectorADSC QUANTUM 4
Wavelength(s)0.97250
Spacegroup nameC 2 2 21
Unit cell lengths80.624, 111.018, 93.297
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution25.000

*

- 2.100
R-factor0.212
Rwork0.212
R-free0.24800

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ir3
RMSD bond length0.006
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.000

*

2.180
High resolution limit [Å]2.1002.100
Rmerge0.048

*

0.254

*

Total number of observations279673

*

Number of reflections24941
Completeness [%]99.9100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.54

*

PEG 8000, sodium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropMg AMP-PCP1 (mM)
31droppeptide1 (mM)
41reservoirPEG800016 (%(w/v))
51reservoirHEPES100 (mM)pH7.5
61reservoir0.15 (M)
71reservoirethylene glycol2 (%(v/v))

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