1K3A
Structure of the Insulin-like Growth Factor 1 Receptor Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97250 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.624, 111.018, 93.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ir3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 * | 0.254 * |
| Total number of observations | 279673 * | |
| Number of reflections | 24941 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG 8000, sodium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Mg AMP-PCP | 1 (mM) | |
| 3 | 1 | drop | peptide | 1 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | 0.15 (M) | ||
| 7 | 1 | reservoir | ethylene glycol | 2 (%(v/v)) |
