1JTY
Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 172.000, 172.000, 95.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.160 - 2.970 |
Rwork | 0.239 |
R-free | 0.29100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1jus |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.160 | 3.150 |
High resolution limit [Å] | 2.970 | 2.970 |
Rmerge | 0.045 | 0.229 |
Number of reflections | 22060 | |
<I/σ(I)> | 12 | 3 |
Completeness [%] | 75.0 | 67.6 |
Redundancy | 5.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |