1JIZ

Crystal Structure Analysis of human Macrophage Elastase MMP-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-01
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	I 2 2 2
Unit cell lengths	67.400, 87.200, 169.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.600
Rwork	0.197
R-free	0.25700 *
RMSD bond length	0.006
RMSD bond angle	1.170 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.600
High resolution limit [Å]	2.600 *	2.550
Rmerge	0.180	0.580
Total number of observations	57864 *
Number of reflections	15776 *
Completeness [%]	100.0	100
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	4 and 20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	20 (mM)	pH7.4
2	1	drop		200 (mM)
3	1	drop		2.5 (mM)
4	1	drop	protein	4 (mg/ml)
5	1	reservoir	sodium cacodylate	100 (mM)	pH6.5
6	1	reservoir	sodium acetate	1.4 (M)