1FYH

1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1995-05-16
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	72.940, 107.600, 85.080
Unit cell angles	90.00, 97.94, 90.00

Refinement procedure

Resolution	15.000 - 2.040
R-factor	0.241
Rwork	0.194
R-free	0.24600
RMSD bond length	0.014
RMSD bond angle	25.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.110
High resolution limit [Å]	2.040	2.040
Rmerge	0.053	0.209
Total number of observations	773440 *
Number of reflections	80837 *
<I/σ(I)>	12.7
Completeness [%]	956.9 *	86.5 *
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	ammonium sulphate, PEG 8000, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2 (M)
2	1	reservoir	PEG8000	8 (%)
3	1	reservoir	Tris-HCl	100 (mM)