1F93
CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THE DIMERIZATION DOMAIN OF HNF-1 ALPHA AND THE COACTIVATOR DCOH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-01 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.480, 82.750, 70.640 |
| Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
| Resolution | 20.100 - 2.600 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 * | 0.243 * |
| Total number of observations | 59400 * | |
| Number of reflections | 16500 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 95.0 | 95 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 4 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 13 (%(w/v)) | |
| 3 | 1 | drop | potassium succinate | 100 (mM) | |
| 4 | 1 | reservoir | PEG400 | 15 (%(v/v)) | |
| 5 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 6 | 1 | reservoir | potassium succinate | 100 (mM) |
