1DGK
MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I WITH GLUCOSE AND ADP IN THE ACTIVE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-02-03 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 145.780, 146.830, 58.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.26 |
| Rwork | 0.258 |
| R-free | 0.31000 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.039 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.064 | 0.440 * |
| Total number of observations | 65575 * | |
| Number of reflections | 25491 * | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 77.5 * | 57 |
| Redundancy | 2.64 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | drop consists of equal volume of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 14-18 (%(w/v)) | precipitant |
| 3 | 1 | reservoir | MES | 200 (mM) | precipitant |
| 4 | 1 | reservoir | sodium acetate | 200 (mM) | precipitant |
| 5 | 1 | reservoir | ADP | 20 (mM) | precipitant |
| 6 | 1 | reservoir | 2 (mM) | precipitant | |
| 7 | 1 | reservoir | 10 (mM) | precipitant | |
| 8 | 1 | reservoir | magnesium acetate | 30 (mM) | precipitant |
