1A4R

G12V MUTANT OF HUMAN PLACENTAL CDC42 GTPASE IN THE GDP FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-01
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	98.650, 98.650, 104.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.225 *
Rwork	0.228
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4Q21 WITH ALL NON-CONSERVED RESIDUES MUTATED TO ALANINE
RMSD bond length	0.007
RMSD bond angle	27.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.049	0.460
Total number of observations	88071 *
Number of reflections	18254 *
<I/σ(I)>	15.6	3.3
Completeness [%]	99.3	99.9
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	15 *	pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mM)
2	1	drop	HEPES-NaOH	20 (mM)
3	1	drop		1 (mM)
4	1	reservoir		0.1 (M)
5	1	reservoir	PEG4000	5 (%)
6	1	reservoir		0.3 (M)